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Flavonoids, natural compounds found in plants, display a broad range of health-beneficial activities. Despite exhibiting various physico-chemical properties and biological activities, their low solubility and stability present challenges. These properties can be improved by effective modification strategy involving introduction of hydrophobic groups onto the flavonoid molecule through the esterification or transesterification reactions. In this study, the synthesis of rutin laurate catalyzed by lipase in bioreactor was investigated. The highest rutin laurate concentration was attained at 1.26 x 10-3 M. The initial esterification rate increased with temperature in the baffled stirrer reactor. The conversions achieved were 49% and 54% at 30 and 45ºC respectively. The esterification process was endothermic with the enthalpy and entropy, values calculated at +51 kJ mol-1 and +102 J mol-1 K-1, respectively. Based on the value of Gibbs free energy change, G the esterification reaction under the conditions studied was predicted to be non-spontaneous below 225°C but spontaneous at higher temperatures. The results revealed in this study demonstrate the broad applicability of bioreactors in the biosynthesis of flavonoid esters. The investigated biochemical process holds practical significance in industrial settings, offering insights for the design and optimization of chemical reactors.