Virtual Booth

This study focused on CRISPR/Cas9 gene knockout system to target the GRA1 gene in the parasite Toxoplasma gondii, which causes severe toxoplasmosis in humans and animals. Since GRA1 is crucial for parasite invasion and survival, knocking it out can reduce its virulence. Using an in-silico (computer-based) approach, we successfully designed and validated three highly efficient single guide RNAs (sgRNAs). These sgRNAs showed excellent on-target precision with zero off-target risks or human gene similarity. These findings offer promising candidates for future therapeutic interventions and treatments against toxoplasmosis.